Vrije Universiteit Brussel

Stationary Phase Development for Shear-Driven Chromatography

To achieve a sufficient retention capacity and mass loadability to be competitive with the traditional HPLC systems, relatively thick micro-porous stationary phase layers need to be arranged on the bottom wall of shear-driven chromatography (SDC) channels. These layers have to be applied with the largest possible pore size and layer thickness uniformity. Theoretical calculations on the influence of deviations from a perfect uniformity will be coupled to experimental measurements. To apply the layers, we will on the one hand use an electrochemical anodization technique for the creation of thin layers of porous silicon and already validated for use in an SDC system. On the other hand, a number of sol-gel deposition techniques for the creation of porous silica-gel and aerogel layers will be explored.

An extensive study of the optimal mobile phase and stationary phase composition will be made for a number of important molecular groups (small uncharged molecules, peptides, oligonucleotides, …)

Vrije Universiteit Brussel

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Stationary Phase Development for Shear-Driven Chromatography