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Contribution to the isolation and hepatic differentiation of postnatal stem/progenitor cells from human adipose tissue

Thursday, 15 December, 2011 - 17:00
Campus: Brussels Health Campus
Faculty: Medicine and Pharmacy
auditorium P. Brouwer
Feras Fayez Abdellatif Albattah
phd defence

Hepatocytes represent the major constituent cells
of the liver and contain the machinery to perform
numerous vital functions, including synthesis of
proteins as well as detoxification of xenobiotics.
These properties render them an attractive in
vitro model for clinical and industrial applications,
in particular when derived from human liver.
Shortage of donor organs, however, represents a
major hurdle to get adequate amounts of human
hepatocytes. Over the past decade, special
attention was paid to generate hepatocytes from
stem cells and progenitor cells from embryonic
and adult origin. In this dissertation, the isolation
of human adipose-derived stem cells (ASCs),
using either a Ficoll density gradient method or a
red blood cell (RBC) lysis buffer, generated the
starting cell material for hepatic differentiation.
First, both cell populations were characterized
with respect to their phenotype, mesodermal and
ectodermal differentiation capacity and
immunomodulating properties. Our results
showed that both isolation methods led to cell
populations that homogeneously expressed
mesenchymal and stemness markers. Moreover,
a substantial difference between both cell
populations was observed in their differentiation
potential towards osteogenic and neurogenic
lineages. Besides their multipotency, in vitro
results demonstrated that hASCs mediated
immunosuppression on T lymphocytes. Regarding
hepatic differentiation, our findings showed that a
small cell fraction of the isolated hASCs can
differentiate into hepatocyte-like cells as
evidenced by immunocytochemistry.

This study demonstrated that hASCs have a
restrictive differentiation capacity towards the
hepatic lineage.